Prevalence of Aeromonas spp. and its Virulence Genes in Samples of Carabeef and Mutton
نویسندگان
چکیده
A survey was conducted to ascertain the distribution and virulence of Aeromonas spp. (species) in carabeef and mutton samples in Greater Hyderabad Municipal Corporation, India. Conventional method using Aeromonas isolation medium and Ampicillin Dextrin agar, PCR targeting 16S rRNA were used to identify Aeromonas spp. in meat samples. Conventional cultural method revealed 28% and 40% positives in mutton and carabeef, respectively whereas PCR assay detected 32% (mutton) and 44% (carabeef) positives suggesting that PCR has a better sensitivity than the cultural method. PCR positives were examined for the presence of toxin genes. Both aerolysin (37.5% in mutton and carabeef) and thermostablecytotonic enterotoxin (36.36% in mutton and 27.27% in carabeef) were detected, indicating the potential pathogenicity of Aeromonas spp. in meat products.
منابع مشابه
Detection of Virulence Genes Tcpa and Ctxb in Isolates from Surface Water and Salt Water Samples in Golestan, Iran
ABSTRACT Background and Objective: Vibrio is a genus of bacteria that are widely distributed in aquatic environments. The genus includes several important pathogens that endanger farm animals and humans who ingest seafood or water contaminated with the bacteria. Virulence of Vibrio spp. is regulated by ctxAB and tcpA genes. The aim of this study...
متن کاملIsolation, Antimicrobial resistance profiles and virulence genotyping of Salmonella spp. from food products in Mazandaran, northern Iran
Background: Salmonella is the most important source of food-borne infections around the world. Human salmonellosis is also found in fresh fruit and vegetable salads. Objective: to investigate detection of Salmonella spp., in Hamburger, Vegetable salads and Cream-filled pastries from factories, supermarkets, restaurants and local confectionery, Mazandaran, Iran, as well as, to assess their pat...
متن کاملComparison of virulence genes in Proteus species isolated from human and pet turtle
The current study was aimed to investigate the prevalence of ureC, rsbA, zapA and mrpA virulence genes using polymerase chain reaction (PCR) in Proteus spp. isolated from 5 commercially popular species of pet turtles and comparison of the mrpA gene sequences of Proteus mirabilis isolates with human clinical isolates. A total of 24 isolates in pet turtles were identified, comprised of P. mirabil...
متن کاملDetection of Zoonotic Antibiotic Resistant Salmonella spp. Carrying Virulence Genes in Rural Mus musculus, Golestan Province, North of Iran
Background and aims: Salmonella spp. infect cold-blooded and warm-blooded animals and maycause a worldwide zoonotic disease, salmonellosis, in infected animals. Rodents can be Salmonellacarriers without any signs of salmonellosis. The frequency of salmonella contamination, the presence ofvirulence genes (SpvR and SpvB) and antibiotic resistance pattern of isolated Salmonella w...
متن کاملA Molecular Survey of Campylobacter jejuni and Campylobacter Coli Virulence and Diversity
Background: The aim of this study was to determine the prevalence of virulence-associated genes and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) analysis of Campylobacter spp. isolated from children with diarrhea in Iran. Methods: A total of 200 stool specimens were obtained from children under 5 years during July 2012 to July 2013. Detection of C. jejuni and C. coli was perfo...
متن کامل